Electrochemical Sensing of DNA Hybridization Based on Duplex-Specific Charge Compensation
نویسندگان
چکیده
منابع مشابه
Electrochemical sensing of DNA hybridization based on duplex-specific charge compensation.
A nonlabeling voltammetric detection method for DNA hybridization has been developed, in which [Fe(CN)(6)](3-) in solution can readily approach an electrode surface covered with a charge-compensated DNA duplex layer and thus provides a strong redox-sensing current. Charge compensation for negative charges on the DNA backbone has been specifically accomplished on DNA duplexes by discouraging non...
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Conducting polymers (CPs) are a group of polymeric materials that have attracted considerable attention because of their unique electronic, chemical, and biochemical properties. This is reflected in their use in a wide range of potential applications, including light-emitting diodes, anti-static coating, electrochromic materials, solar cells, chemical sensors, biosensors, and drug-release syste...
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DNA conjugates containing adjacent duplex and guanine quadruplex assemblies have been designed to explore charge transport into quadruplex architectures. The quadruplex assemblies have been characterized structurally using circular dichroism and by assaying for chemical protection. Using an intercalating rhodium photooxidant, noncovalently bound or tethered to the duplex end, oxidizing radicals...
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We report on real-time electrochemical detection of individual DNA hybridization events at an electrode surface. The experiment is carried out in a microelectrochemical device configured with a working electrode modified with single-stranded DNA probe molecules. When a complementary DNA strand labelled with a catalyst hybridizes to the probe, an easily detectable electrocatalytic current is obs...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2005
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac051536b